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Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)
Version of Record online: 21 SEP 2010
© 2010 The Authors. Insect Molecular Biology © 2010 The Royal Entomological Society
Insect Molecular Biology
Volume 20, Issue 1, pages 105–114, February 2011
How to Cite
Kamau, L., Skilton, R. A., Odongo, D. O., Mwaura, S., Githaka, N., Kanduma, E., Obura, M., Kabiru, E., Orago, A., Musoke, A. and Bishop, R. P. (2011), Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida). Insect Molecular Biology, 20: 105–114. doi: 10.1111/j.1365-2583.2010.01043.x
- Issue online: 7 JAN 2011
- Version of Record online: 21 SEP 2010
Figure S1. PCR amplification of Ra86 from gut RNA from 20 individual female R. appendiculatus ticks. Controls: PCR amplification of cDNA plasmids representing Ra86-4 (clone 85A) and Ra86-5 (clone 92A), respectively. The predicted sizes of the Ra86-4 and Ra86-5 PCR products are 890 bp and 1010 bp, respectively. Molecular markers for sizes indicated (lane M).
Figure S2. Alignment of Ra86 3′ untranslated region sequences.
Table S1. GenBank accession numbers of 19 Ra86 homologues of the Bm86 antigen isolated from Rhipicephalus appendiculatus.
Table S2. Summary of sequence similarity of mitochondrial cytochrome oxidase 1 sequences representing a consensus sequence derived from individual ticks expressing Ra86-4 and Ra86-5 in gut cDNA. These were searched against the GenBank nr sequence database using blastn.
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