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Figure S1. The RNA interference efficacy and potential off-target were examined at 72 h. Chace1 or Chace2 was knocked down specifically with a negligible effect on another ace gene.

Figure S2. Remained enzyme activities measured at 96 h after small interfering RNA (siRNA) injection.

Figure S3. The mortalities of siRNA-treated rice stem borers at 24, 48, 72 and 96 h.

Figure S4. Average larvae weight of siRNA-treated rice stem borer at 7 days, 14 days, and 21 days.

Figure S5. Average larvae body lengths of siRNA-treated rice stem borer at 7 days, 14 days, and 21 days.

Video S1. Movement of siRNA-treated rice stem borers. The Csace1-silenced and Csace1& Csace2- silenced rice stem borers moved more slowly than the controls.

Video S2. Feeding test of siRNA-treated rice stem borers. The Csace1- silenced and Csace1& Csace2- silenced rice stem borers failed to reach the germinated rice seed.

FilenameFormatSizeDescription
IMB_1081_sm_FS1-5.doc218KSupporting info item
IMB_1081_sm_VideoS1.wmv3552KSupporting info item
IMB_1081_sm_VideoS2.wmv5092KSupporting info item

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