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Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus

Authors

  • T. Watanabe,

    1. Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan
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  • H. Sadamoto,

    1. Laboratory of Functional Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Sanuki, Japan
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  • H. Aonuma

    Corresponding author
    1. Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan
      Hitoshi Aonuma, Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, Hokkaido University, Nishi 7 Chome, Kita 12 Jo, Kitaku, Sapporo, Hokkaido 060-0812, Japan. Tel.: +81-11-706-3832; fax: +81-11-706-3832; e-mail: aon@es.hokudai.ac.jp
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Hitoshi Aonuma, Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, Hokkaido University, Nishi 7 Chome, Kita 12 Jo, Kitaku, Sapporo, Hokkaido 060-0812, Japan. Tel.: +81-11-706-3832; fax: +81-11-706-3832; e-mail: aon@es.hokudai.ac.jp

Abstract

Serotonin (5-HT) modulates various aspects of behaviours such as aggressive behaviour and circadian behaviour in the cricket. To elucidate the molecular basis of the cricket 5-HT system, we identified 5-HT-related genes in the field cricket Gryllus bimaculatus DeGeer. Complementary DNA of tryptophan hydroxylase and phenylalanine-tryptophan hydroxylase, which convert tryptophan into 5-hydroxy-L-tryptophan (5-HTP), and that of aromatic L-amino acid decarboxylase, which converts 5-HTP into 5-HT, were isolated from a cricket brain cDNA library. In addition, four 5-HT receptor genes (5-HT1A, 5-HT1B, 5-HT, and 5-HT7) were identified. Expression analysis of the tryptophan hydroxylase gene TRH and phenylalanine-tryptophan hydroxylase gene TPH, which are selectively involved in neuronal and peripheral 5-HT synthesis in Drosophila, suggested that two 5-HT synthesis pathways co-exist in the cricket neuronal tissues. The four 5-HT receptor genes were expressed in various tissues at differential expression levels, suggesting that the 5-HT system is widely distributed in the cricket.

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