Figure S1. Schematic representation of protein structure of two Gryllus TRH isoforms. Gryllus TRH long variant contains a 39 amino acid (117 bp) residue insertion within the N-terminal region of the catalytic domain.

Figure S2. Tissue-specific expression patterns of three house keeping genes (Gryllus ß-actin, Ef1alpha, and rp49). Ef1alpha expression was the one that was most stable in the different tissues.

Figure S3. Tissue-specific expression patterns of Gryllus TRH, TPH, 5-HT, and 5-HT7 genes. The PCR products of a secondary PCR were run through a 1.5% agarose gel and visualized by ethidium bromide. Gryllus Ef1alpha gene was used as a internal control gene.

IMB_1093_sm_supp_f1.eps454KSupporting info item
IMB_1093_sm_supp_f2.eps2606KSupporting info item
IMB_1093_sm_supp_f3.eps3219KSupporting info item

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