Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus
Article first published online: 24 JUN 2011
© 2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society
Insect Molecular Biology
Volume 20, Issue 5, pages 619–635, October 2011
How to Cite
Watanabe, T., Sadamoto, H. and Aonuma, H. (2011), Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus. Insect Molecular Biology, 20: 619–635. doi: 10.1111/j.1365-2583.2011.01093.x
- Issue published online: 19 SEP 2011
- Article first published online: 24 JUN 2011
Figure S1. Schematic representation of protein structure of two Gryllus TRH isoforms. Gryllus TRH long variant contains a 39 amino acid (117 bp) residue insertion within the N-terminal region of the catalytic domain.
Figure S2. Tissue-specific expression patterns of three house keeping genes (Gryllus ß-actin, Ef1alpha, and rp49). Ef1alpha expression was the one that was most stable in the different tissues.
Figure S3. Tissue-specific expression patterns of Gryllus TRH, TPH, 5-HT2α, and 5-HT7 genes. The PCR products of a secondary PCR were run through a 1.5% agarose gel and visualized by ethidium bromide. Gryllus Ef1alpha gene was used as a internal control gene.
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