Figure S1. Southern analysis of Aedes aegypti heat shock protein 70A-1373 (AaHsp70A-1373) and AaHsp70B-1456 in Mos1 transformed Ae. aegypti. (A, C) Hypothetical representation of AaHsp70Aa-1373-firefly luciferase (AaHsp70Aa-1373-FFluc) and AaHsp70Bb-1456-FFluc transgene insertions. Block arrows represent right (R) and left (L) arms of the Mos1 transposon. Dashed lines indicate Ae. aegypti genomic DNA. Predicted restriction sites for EcoRI (E), HindIII (H), and NdeI (N) are indicated. Bracketed bars indicate the size of the entire insertion as well as the expected size of one of the bands resulting from HindIII digestion. (B, D) Genomic DNA from wild type (wt) or transgenic mosquitoes was hybridized with a probe corresponding to the Mos1 right arm and DsRed cassette. Molecular weight markers are indicated to the left of each image. For (B), black arrows indicate the internal HindIII fragment, which should be common to all insertions, white arrows indicate junction fragments with mosquito genomic DNA. For (C), white arrows again indicate junction fragments with mosquito genomic DNA. All transgenic lines appear to contain only a single insertion, with the exception of line #P3. In this case, the EcoRI digest produced two strong hybridization fragments, whereas the NdeI digest only produced one. Thus, it is not clear if there are one or two insertions in this line.

Table S1.Mos1-mediated transformation of Aedes aegypti with Ae. aegypti heat shock protein 70-firefly luciferase (AaHsp70-FFluc) constructs.

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