Current Address: Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06510, USA
Molecular characterization of Ephestia kuehniella (Lepidoptera: Pyralidae) transferrin and its response to parasitoid Venturia canescens (Hymenoptera: Ichneumonidae Gravenhorst)
Article first published online: 9 JAN 2012
© 2012 The Authors. Insect Molecular Biology © 2012 The Royal Entomological Society
Insect Molecular Biology
Volume 21, Issue 2, pages 139–147, April 2012
How to Cite
Guz, N., Kilincer, N. and Aksoy, S. (2012), Molecular characterization of Ephestia kuehniella (Lepidoptera: Pyralidae) transferrin and its response to parasitoid Venturia canescens (Hymenoptera: Ichneumonidae Gravenhorst). Insect Molecular Biology, 21: 139–147. doi: 10.1111/j.1365-2583.2011.01129.x
Preliminary data of this study has been published as an abstract in Proceedings of the Third Plant Protection Congress of Turkey, 2009.
- Issue published online: 8 MAR 2012
- Article first published online: 9 JAN 2012
- Ephestia kuehniella;
- Venturia canescens;
- defence reactions
In the present study, we characterized a full-length cDNA encoding a putative iron-binding protein transferrin from the lepidopteran Mediterranean flour moth (EkTrf, 2397 bp). The putative EkTrf is 683 amino acids with a molecular mass of approximately 76 kDa. The deduced amino acid sequence showed significant homology with other insect transferrins from Chilo suppressalis (76%), Galleria mellonella (75%), Plutella xylostella (72%), Manduca sexta (74%), Bombyx mori (73%), Spodoptera litura and (72%), Choristoneura fumiferana (71%). Northern blot analysis indicated that Ephestia transferrin mRNA was expressed in the last larval instars of both males and females and in the pupal developmental stages. EkTrf is expressed predominantly in the fat body and ovary tissues. Analysis of parasitized larva by the endoparasitoid Venturia canescens suggests that transferrin expression is induced following parasitoid challenge. Expression of EkTrf levels also increased upon bacterial infection at 6 h post treatment and remained high until 24 h. Similarly to other insect transferrins, EkTrf may play a role in immunity through its iron-binding capacity.