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Figure S1. Gene expression levels in healthy and pathogen-exposed Megachile rotundata larvae at different temperatures. Positive points (red circles) are genes with greater expression levels in pathogen-exposed larvae than in healthy larvae at the given temperature. Negative points (green triangles) are genes with lower expression levels in pathogen-exposed larvae than in healthy larvae at the given temperature. Dark circles indicate genes expressed at similar levels in both healthy and pathogen-exposed larvae. H-P total is the gene expression of pathogen-exposed larvae relative to that of healthy larvae when the data are combined across all temperatures. The data were analysed using SAM software with δ = 0.3.

Figure S2. Relative gene expression for healthy Megachile rotundata larvae when each temperature is compared with a higher temperature. Positive points (red circles) are genes in healthy larvae that were up-regulated when rearing temperature increased, and negative points (green triangles) are genes that were down-regulated when the temperature increased. The data were analysed using SAM software with δ = 0.3.

Figure S3. Relative gene expression for pathogen-exposed Megachile rotundata larvae when each rearing temperature is compared with a higher rearing temperature. Positive points (red circles) are genes in pathogen-exposed larvae that were up-regulated when rearing temperature increased, and negative points (green triangles) are genes that were down-regulated when the temperature increased. The data were analysed using SAM software with δ = 0.3.

Table S1. The sequences and characteristics of the triple-oligonucleotide probes.

FilenameFormatSizeDescription
IMB_1133_sm_FS1.pdf99KSupporting info item
IMB_1133_sm_FS2.pdf95KSupporting info item
IMB_1133_sm_FS3.pdf94KSupporting info item
IMB_1133_sm_TS1.pdf377KSupporting info item

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