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Figure S1. The coefficient of variation decreases with increasing spectral count. Coefficient of variation and median spectral count were calculated based on seven technical replicates of an abdominal sample from Bombus impatiens. The red vertical line indicates a cut-off at a median spectral count of seven.

Figure S2. The percentage of proteins with a coefficient of variation smaller or equal to 0.5 increases with increasing spectral count. Coefficient of variation and median spectral count were calculated based on seven technical replicates of an abdominal sample from Bombus impatiens. The red vertical line indicates a cut-off at a median spectral count of seven (the cut-off selected in this study), at which over 90% of all proteins exhibit a coefficient of variation ≤ 0.5.

Table S1. The table contains two worksheets. The first lists proteins quantified in Bombus terrestris brain samples. It shows accession numbers, values used in the analysis and statistical details including P-values, medians, coefficients of variation and rank sums. The second contains the raw spectral count information for all B. terrestris brain samples before replacement of missing values (NAs) and normalization. Isoforms of the same protein are differentiated by their accession numbers but can share the same name and the same peptides.

Table S2. The table contains two worksheets. The first lists proteins quantified in Bombus impatiens brain samples. It lists accession numbers, values used in the analysis and statistical details including P-values, medians, coefficients of variation and rank sums. The second contains the raw spectral count information for all B. terrestris brain samples before replacement of missing values (NAs) and normalization. Isoforms of the same protein are differentiated by their accession numbers but can share the same name and the same peptides.

Table S3. The table contains two worksheets. The first lists proteins quantified in Bombus impatiens abdominal tissue samples. It lists accession numbers, values used in the analysis and statistical details including P-values, medians, coefficients of variation and rank sums. The second contains the raw spectral count information for all B. terrestris brain samples before replacement of missing values (NAs) and normalization. Isoforms of the same protein are differentiated by their accession numbers but can share the same name and the same peptides.

FilenameFormatSizeDescription
IMB_1142_sm_file1.doc519KSupporting info item
IMB_1142_sm_tableS1.xls726KSupporting info item
IMB_1142_sm_tableS2.xls846KSupporting info item
IMB_1142_sm_tableS3.xls856KSupporting info item

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