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Figure S1. Survival rate after double-stranded RNA (dsRNA) treatments in fifth nymphal locusts. Nymphs (both male and female 50/50) were injected on the second day after their moult with 1 μg (n = 30), 0.5 μg (n = 30) and 0.25 μg (n = 30) clk dsRNA or 0.5 μg gfp dsRNA (n = 31). The different plots represent the percentage of locusts alive at a given time after the first dsRNA injection. Log rank test for survival indicates that survival between all conditions of one experiment was significantly different.

Figure S2. Relative transcript levels of clock (clk) after clk double-stranded RNA (dsRNA) treatment in brains of 4-day-old females. Fifth instar nymphs were injected on the second day after their moult with 1 μg clk or green fluorescent protein (gfp) dsRNA. Statistical significance (Mann−Whitney, *** for P < 0.001) and standard error are shown on top of the column bars. n = 6 for each condition.

Figure S3. Effect of double-stranded RNA (dsRNA) treatment on size of the fat body (A) and ovary weight (B). Fifth instar nymphs were injected on the second day after their moult with 0.25 μg clock (clk) or green fluorescent protein (gfp) dsRNA. At day 10 of adulthood, the ovaries were dissected from treated locusts and weighed to assess the effect of dsRNA treatment on their development (n = 9). During the dissection, the cuticle of the abdomen was photographed to show the developed fat body in the abdomen. Statistical significance (Mann−Whitney, *** for P < 0.001) and standard error are shown on top of the column bars.

FilenameFormatSizeDescription
IMB_1143_sm_FigS1.tif20014KSupporting info item
IMB_1143_sm_FigS2.tif16587KSupporting info item
IMB_1143_sm_FigS3.tif2349KSupporting info item

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