RNA interference mortality points to noncircadian functions for the clock gene in the desert locust Schistocerca gregaria
Article first published online: 20 MAR 2012
© 2012 The Authors. Insect Molecular Biology © 2012 The Royal Entomological Society
Insect Molecular Biology
Volume 21, Issue 3, pages 369–381, June 2012
How to Cite
Tobback, J., Vuerinckx, K., Boerjan, B. and Huybrechts, R. (2012), RNA interference mortality points to noncircadian functions for the clock gene in the desert locust Schistocerca gregaria. Insect Molecular Biology, 21: 369–381. doi: 10.1111/j.1365-2583.2012.01143.x
- Issue published online: 8 MAY 2012
- Article first published online: 20 MAR 2012
Figure S1. Survival rate after double-stranded RNA (dsRNA) treatments in fifth nymphal locusts. Nymphs (both male and female 50/50) were injected on the second day after their moult with 1 μg (n = 30), 0.5 μg (n = 30) and 0.25 μg (n = 30) clk dsRNA or 0.5 μg gfp dsRNA (n = 31). The different plots represent the percentage of locusts alive at a given time after the first dsRNA injection. Log rank test for survival indicates that survival between all conditions of one experiment was significantly different.
Figure S2. Relative transcript levels of clock (clk) after clk double-stranded RNA (dsRNA) treatment in brains of 4-day-old females. Fifth instar nymphs were injected on the second day after their moult with 1 μg clk or green fluorescent protein (gfp) dsRNA. Statistical significance (Mann−Whitney, *** for P < 0.001) and standard error are shown on top of the column bars. n = 6 for each condition.
Figure S3. Effect of double-stranded RNA (dsRNA) treatment on size of the fat body (A) and ovary weight (B). Fifth instar nymphs were injected on the second day after their moult with 0.25 μg clock (clk) or green fluorescent protein (gfp) dsRNA. At day 10 of adulthood, the ovaries were dissected from treated locusts and weighed to assess the effect of dsRNA treatment on their development (n = 9). During the dissection, the cuticle of the abdomen was photographed to show the developed fat body in the abdomen. Statistical significance (Mann−Whitney, *** for P < 0.001) and standard error are shown on top of the column bars.
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