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imb1162-sup-0001-si.pdf3135K

Figure S1. The dissection strategy used to isolate tissues before RNA extraction for primary cDNA library construction. First, the mouthparts including the antennae and the suboesophageal ganglion were removed from the mandibular side of soldier heads. The brain was removed next. The remaining head containing class 1 gland cell layer and class 3 gland cells was used for RNA extraction.

imb1162-sup-0001-si.pdf3135K

Figure S2. cDNA sequences and deduced amino acid sequences of the NtHMGS protein (1 785 base pairs, open reading frame encoding 472 amino acid residues) obtained by GS Junior and 3′-rapid amplification of cDNA ends (3′RACE) methods. The deduced amino acid sequences are shown below the nucleotide sequences. The termination codons are indicated by asterisks. The possible polyadenylation signals are double underlined. Dotted arrows indicate the region of the primer used for 3′-RACE. The region used for real-time quantitative reverse-transcriptase PCR is underlined, and the regions of the primers are indicated by arrows.

imb1162-sup-0001-si.pdf3135K

Figure S3. cDNA sequences and deduced amino acid sequences of the NtHMGR protein (3 241 base pairs, open reading frame encoding 869 amino acid residues) obtained by GS Junior and 3′-rapid amplification of cDNA ends (3′-RACE) methods. The deduced amino acid sequences are shown below the nucleotide sequences. The termination codons are indicated by asterisks. Dotted arrows indicate the region of the primer used for 3′-RACE. The region used for real-time quantitative reverse-transcriptase PCR is underlined, and the regions of the primers are indicated by arrows.

imb1162-sup-0001-si.pdf3135K

Figure S4. Comparison of the deduced amino acid sequence of NtHMGS with those of HMGS proteins retrieved from insects Blattella germanica (GenBank accession nos. P54961 and P54870), Tribolium castaneum (XP_973437), Drosophila melanogaster (NP_524711), Aedes aegypti (XP_001650491), Culex quinquefasciatus (XP_001843333), and Nasonia vitripennis (XP_003426942) and other animals Pongo abelii (NP_001127508), Sus scrofa (XP_003133967), Monodelphis domestica (XP_001364426), Homo sapiens (NP_001091742), Anolis carolinensis (XP_003216245), and Saccoglossus kowalevskii (XP_002738314). Completely identical, conservative, and semi-conservative amino acid residues are indicated with ‘*,’ ‘:,’ and ‘.,’ respectively. The characteristic highly conserved amino acid residues are shaded grey.

imb1162-sup-0001-si.pdf3135K

Figure S5. Comparison of the deduced amino acid sequence of NtHMGR with HMGR proteins retrieved from insects Blattella germanica (GenBank accession no. P54960), Pediculus humanus corporis (XP_002428525), Tribolium castaneum (XP_973850), Phaedon cochleariae (ABO37160), Apis mellifera (XP_623118), Nasonia vitripennis (XP_001601404), Camponotus floridanus (EFN61977), Harpegnathos saltator (EFN88878), Ips paraconfusus (AAD20975), Dendroctonus jeffreyi (AAF80374), and Anthonomus grandis (AAF80475); and vertebrates Danio rerio (AAI55136), Taeniopygia guttata (XP_002187727), Nomascus leucogenys (XP_003266121), Monodelphis domestica (XP_001368743), and Cricetulus griseus (XP_003508040). Completely identical, conservative, and semi-conservative amino acid residues are indicated with ‘*,’ ‘:,’ and ‘.,’ respectively. The characteristic highly conserved amino acid residues are shaded grey.

imb1162-sup-0001-si.pdf3135K

Figure S6. Partitions of soldier heads used for real-time quantitative reverse-transcriptase PCR. The diagram is drawn in cross-section. Dotted lines indicate separated lines. The nasus (NA) part includes class 3 gland cells situated along the path of secretion. The frontal gland (FG) part includes the class 1 gland cell layer around the frontal gland reservoir. The mouthparts and brain (MB) part includes the suboesophageal ganglion and brain. Antennae (AN) and body (BO; thorax and abdomen) were also separated.

imb1162-sup-0002-si.xlsx170K

Table S1. Genes (isogroups) assembled by Newbler (ver 2.5p1)

imb1162-sup-0002-si.xlsx170K

Table S2. Contigs assembled by Newbler (ver 2.5p1)

imb1162-sup-0002-si.xlsx170K

Table S3. Primer sets for real-time quantitative reverse-transcriptase PCR

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