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imb1163-sup-0001-si.pdf358K

Figure S1. Multiple sequence alignment of Orseolia oryzae nucleoside diphosphate kinase (OoNDPK) showing homology to NDPKs from different insects. Arrow shows amino acid residues that form the conserved domain [N-X-X-H-(GA)-S-D].

imb1163-sup-0002-si.zip247K

Figure S2. Signal sequence and most likely cleavage site of Orseolia oryzae nucleoside diphosphate kinase (OoNDPK) predicted using two different prediction models. (A) SignalP-NN prediction (eukaryotic networks). (B) SignalP-HMM prediction (eukaryotic model). Both models predicted cleavage site between the 17th and 18th residues (http://www.cbs.dtu.dk/services/SignalP/).

imb1163-sup-0003-si.pdf1173K

Figure S3. Coleoptile cell elongation assay. Jaya seeds (72 h post-germination) were treated with recombinant Orseolia oryzae nucleoside diphosphate kinase (OoNDPK; see Experimental procedures). Coleoptile cells were observed under a light microscope 24 h after exposure to (A) water; (B) heat-denatured protein (recombinant OoNDPK); and (C) recombinant protein (OoNDPK). Scale bar = 20 μm.

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