Reduction of malaria transmission by transgenic mosquitoes expressing an antisporozoite antibody in their salivary glands
Article first published online: 26 NOV 2012
© 2012 Royal Entomological Society
Insect Molecular Biology
Volume 22, Issue 1, pages 41–51, February 2013
How to Cite
Sumitani, M., Kasashima, K., Yamamoto, D. S., Yagi, K., Yuda, M., Matsuoka, H. and Yoshida, S. (2013), Reduction of malaria transmission by transgenic mosquitoes expressing an antisporozoite antibody in their salivary glands. Insect Molecular Biology, 22: 41–51. doi: 10.1111/j.1365-2583.2012.01168.x
- Issue published online: 18 JAN 2013
- Article first published online: 26 NOV 2012
- Grant-in-Aid for Young Scientists (B). Grant Number: 22790398
- Grant-in-Aid for Scientific Research (B). Grant Number: 21390126
Figure S1. The DNA sequence and its deduced amino acid sequence of the variable region of light chain (VL) and heavy chain (VH). Nucleotide numbers are shown on the left.
Figure S2. Fluorescence microscopy (upper) and transmission (lower) images of the salivary glands of a transgenic mosquito. (A, C) Salivary gland from a 1-day-old transgenic female mosquito. (B, D) Salivary gland from a male transgenic mosquito. Scale bar = 100 μm. (E) Detection of the mDsRed-2A10 scFv in a 1-day-old adult transgenic mosquito. A 56-kDa band was detected in salivary glands, but not in carcasses following salivary gland removal.
Table S1. (A) Number of wild-type (WT) mosquitoes fed on individual naïve mice. (B) Number of transgenic (TG) mosquitoes fed on individual naïve mice.
Supplemental material. The DNA sequence of the aappP-mDsRed-2A10scFv-Antryp1 gene cassette.
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