Trans-enamel and trans-dentinal cytotoxic effects of a 35% H2O2 bleaching gel on cultured odontoblast cell lines after consecutive applications

  1. F. Z. Trindade1,
  2. A. P. D. Ribeiro1,
  3. N. T. Sacono2,
  4. C. F. Oliveira2,
  5. F. C. R. Lessa2,
  6. J. Hebling2,
  7. C. A. S. Costa3

Article first published online: 28 APR 2009

DOI: 10.1111/j.1365-2591.2009.01544.x

Issue

International Endodontic Journal

International Endodontic Journal

Volume 42, Issue 6, pages 516–524, June 2009

Additional Information

How to Cite

Trindade, F. Z., Ribeiro, A. P. D., Sacono, N. T., Oliveira, C. F., Lessa, F. C. R., Hebling, J. and Costa, C. A. S. (2009), Trans-enamel and trans-dentinal cytotoxic effects of a 35% H2O2 bleaching gel on cultured odontoblast cell lines after consecutive applications. International Endodontic Journal, 42: 516–524. doi: 10.1111/j.1365-2591.2009.01544.x

Author Information

  1. 1

    Departments of Dental Materials and Prosthodontics

  2. 2

    Orthodontics and Pediatric Dentistry

  3. 3

    Physiology and Pathology, Araraquara School of Dentistry, São Paulo State University, Araraquara, SP, Brazil

*Prof. Dr Carlos Alberto de Souza Costa, Departamento de Fisiologia e Patologia, Faculdade de Odontologia de Araraquara, Universidade Estadual Paulista, Rua Humaitá, 1680, Centro, Caixa Postal: 331, Cep: 14801903 Araraquara, SP, Brazil (Tel.: +55-16-3301-6477; fax: +55-16-3301-6488; e-mail: casouzac@foar.unesp.br).

Publication History

  1. Issue published online: 28 APR 2009
  2. Article first published online: 28 APR 2009
  3. Received 14 August 2008; accepted 23 December 2008
Corrected by:

Erratum

Vol. 42, Issue 7, 649, Article first published online: 1 JUN 2009

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Keywords:

  • cytotoxicity;
  • hydrogen peroxide;
  • odontoblasts;
  • tooth bleaching

Abstract

Aim  To evaluate the trans-enamel and trans-dentinal cytotoxic effects of a 35% H2O2 bleaching gel on an odontoblast-like cell lines (MDPC-23) after consecutive applications.

Methodology  Fifteen enamel/dentine discs were obtained from bovine central incisor teeth and placed individually in artificial pulp chambers. Three groups (n = 5 discs) were formed according to the following enamel treatments: G1: 35% H2O2 bleaching gel (15 min); G2: 35% H2O2 bleaching gel (15 min) + halogen light (20 s); G3: control (no treatment). After repeating the treatments three consecutive times, the extracts (culture medium + gel components that had diffused through enamel/dentine discs) in contact with the dentine were collected and applied to previously cultured MDPC-23 cells (50 000 cells cm−2) for 24 h. Cell metabolism was evaluated by the MTT assay and data were analysed statistically (α = 5%; Kruskal–Wallis and Mann–Whitney U-test). Cell morphology was analysed by scanning electron microscopy.

Results  Cell metabolism decreased by 92.03% and 82.47% in G1 and G2 respectively. G1 and G2 differed significantly (P < 0.05) from G3. Regardless of halogen light activation, the application of the bleaching gel on the cultured odontoblast-like cells caused significantly more severe cytotoxic effects than those observed in the nontreated control group. In addition, significant morphological cell alterations were observed in G1 and G2.

Conclusion  After three consecutive applications of a 35% H2O2 bleaching agent, the diffusion of the gel components through enamel and dentine caused severe toxic effects to cultured pulp cells.

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