Viability of human fibroblasts in coconut water as a storage medium

  1. J. J. S. Moreira-Neto1,
  2. J. O. Gondim2,
  3. M. S. G. Raddi3,
  4. C. A. Pansani2

Article first published online: 22 JUN 2009

DOI: 10.1111/j.1365-2591.2009.01591.x

Issue

International Endodontic Journal

International Endodontic Journal

Volume 42, Issue 9, pages 827–830, September 2009

Additional Information

How to Cite

Moreira-Neto, J. J. S., Gondim, J. O., Raddi, M. S. G. and Pansani, C. A. (2009), Viability of human fibroblasts in coconut water as a storage medium. International Endodontic Journal, 42: 827–830. doi: 10.1111/j.1365-2591.2009.01591.x

Author Information

  1. 1

    Department of Dental Clinics, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil

  2. 2

    Department of Pediatric Dentistry, School of Dentistry of Araraquara, São Paulo State University, Araraquara, SP, Brazil

  3. 3

    Department of Clinical Analyses, School of Pharmaceutical Sciences, São Paulo State University, Araraquara, SP, Brazil

*Juliana Oliveira Gondim, Rua Av. Cel. Miguel Dias, 372 – Edson Queiroz, CEP 60810-160, Fortaleza, CE, Brazil (Tel.: +55 85 8895 2075; e-mail: jujugondim@yahoo.com.br)

Publication History

  1. Issue published online: 6 AUG 2009
  2. Article first published online: 22 JUN 2009
  3. Received 16 November 2008; accepted 2 April 2008

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Keywords:

  • avulsion;
  • coconut water;
  • human fibroblasts;
  • storage media

Abstract

Aim  To evaluate the effectiveness of a new storage medium for avulsed teeth, coconut water, in maintaining the viability of human fibroblasts.

Methodology  Cell viability after different time periods was evaluated in the following storage media: coconut water, coconut water with sodium bicarbonate, milk, saline and still mineral water. Human fibroblasts were seeded in Eagle’s minimal essential medium (EMEM) supplemented with 7.5% foetal calf serum. After trypsinisation, 100 μL of culture medium containing approximately 104 cells mL−1 were collected and pipetted into the wells of 96-well plates, which were incubated overnight in 5% CO2 and 95% air mixture at 37 °C. EMEM was then replaced by the storage media and the plates were incubated at 37 °C for 1, 2 and 4 h. Cell viability was determined using the neutral red assay. The proportions of viable cells after exposure to the storage media were analysed statistically by anova and the least significant difference (LSD) test (α = 5%).

Results  Milk had the greatest capacity to maintain cell viability (P < 0.05), followed by coconut water with sodium bicarbonate and saline. Coconut water was significantly worse at maintaining cell viability compared to milk, coconut water with sodium bicarbonate and saline. The smallest number of viable cells was observed for mineral water (P < 0.05).

Conclusion  Coconut water was worse than milk in maintaining human fibroblast cell viability.

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