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Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis

Authors


Mário Tanomaru-Filho, Rua Humaitá, 1680, Caixa Postal 331, Centro, 14801-903 Araraquara, SP, Brazil (Tel.: +55-16-3301-6390; fax: +55-16-3301-6392; e-mail: tanomaru@uol.com.br).

Abstract

Lima RKP, Guerreiro-Tanomaru JM, Faria-Júnior NB, Tanomaru-Filho M. Effectiveness of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis. International Endodontic Journal, 45, 311–316, 2012.

Abstract

Aim  To evaluate the antimicrobial efficacy of calcium hydroxide-based intracanal medicaments against Enterococcus faecalis.

Methodology  One hundred and six single-rooted human teeth were inoculated with E. faecalis (ATCC 29212) and incubated in an microaerophilic environment for 21 days. Teeth were divided according to the medicaments and period: G1 – Calen (7 days); G2 – Calen (14 days); G3 – Calen/camphorated paramonochlorophenol (CMCP) (7 days); G4 – Calen/CMCP (14 days); G5 – Calen/0.4% chlorhexidine (CHX) (7 days); G6 – Calen/0.4% CHX (14 days); G7 – Calen/1% CHX (7 days); G8 – Calen/1% CHX (14 days); G9 – chemo-mechanical preparation with no medication and G10 – no chemo-mechanical preparation and no medication. Microbial samples were harvested immediately after removal of the intracanal dressing and 7 days later. Data were subjected to anova (P < 0.05).

Results  All medicaments were associated with a reduction in E. faecalis after removal of the dressing (post-medication harvest). However, all specimens were associated with an increase in CFU mL−1 values after 7 days (final harvest). Calen/CMCP and Calen/CHX had the lowest CFU mL−1 values (P < 0.05).

Conclusion  In this laboratory study using extracted teeth all calcium hydroxide-based medicaments were able to significantly reduce the CFU mL−1 values of E. faecalis in the root canal system.

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