Tenascin-C promotes differentiation of rat dental pulp cells in vitro
Article first published online: 30 JUN 2012
© 2012 International Endodontic Journal
International Endodontic Journal
Volume 46, Issue 1, pages 30–39, January 2013
How to Cite
Matsuoka K, Matsuzaka K, Yoshinari M & Inoue T. Tenascin-C promotes differentiation of rat dental pulp cells in vitro. International Endodontic Journal, 46, 30–39, 2013.
- Issue published online: 9 DEC 2012
- Article first published online: 30 JUN 2012
- Accepted manuscript online: 1 JUN 2012 01:39PM EST
- Manuscript Accepted: 27 MAY 2012
- Manuscript Received: 19 JAN 2012
- Grant HRC7 from the Oral Health Science Center of the Tokyo Dental College
- ‘High-Tech Research Center’
- dental pulp cells;
- extracellular matrix;
To investigate the effects of tenascin-C (TN-C) on cultured rat dental pulp cells in relation to the expression of Notch signalling.
Subcultured dental pulp cells derived from rat incisors were seeded both in wells and on plastic coverslips coated with various concentrations of recombinant human TN-C. Expression of bone-related mRNA was then analysed by RT-PCR and observed by immunohistochemical staining. Encoding of Notch1 and Notch2 (markers of initial differentiation of odontoblast-like cells), alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) (markers of mineralization) was investigated. Non-TN-C-coated wells were used as controls. Primary antibodies to Notch1, ALP and OCN were used for immunofluorescence staining, and ALP activity was evaluated. Data were compared using Student's t-test.
Cell proliferation rate in the experimental groups was significantly higher (P < 0.05) than that in the control group at 72 h. Expression of Notch1, Notch2, ALP, OPN and OCN mRNAs was significantly higher (P < 0.05) in the experimental group than that in the control group. Strongly positive staining for Notch1, ALP and OCN was observed in the experimental group. ALP activity was significantly higher (P < 0.01) in the experimental group than in the control group at 24 h.
TN-C promoted differentiation of rat dental pulp cells by the activation of Notch.