All authors meet ICMJE authorship criteria. Nobody who qualifies for authorship has been excluded.
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ORIGINAL ARTICLE
Gene expression profiling in male genital lichen sclerosus
Article first published online: 30 JUN 2011
DOI: 10.1111/j.1365-2613.2011.00779.x
© 2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology
Issue
International Journal of Experimental Pathology
Volume 92, Issue 5, pages 320–325, October 2011
Additional Information
How to Cite
Edmonds, E., Barton, G., Buisson, S., Francis, N., Gotch, F., Game, L., Haddad, M., Dinneen, M. and Bunker, C. (2011), Gene expression profiling in male genital lichen sclerosus. International Journal of Experimental Pathology, 92: 320–325. doi: 10.1111/j.1365-2613.2011.00779.x
Publication History
- Issue published online: 14 SEP 2011
- Article first published online: 30 JUN 2011
- Received for publication: 29 December 2010 Accepted for publication: 7 May 2011
- Abstract
- Article
- References
- Cited By
Keywords:
- gene expresssion profiling;
- male genital lichen sclerosus;
- squamous carcinoma
Summary
Male genital lichen sclerosus (MGLSc) has a bimodal distribution in boys and men. It is associated with squamous cell carcinoma (SCC). The pathogenesis of MGLSc is unknown. HPV and autoimmune mechanisms have been mooted. Anti extracellular matrix protein (ECM)1 antibodies have been identified in women with GLSc. The gene expression pattern of LSc is unknown. Using DNA microarrays we studied differences in gene expression in healthy and diseased prepuces obtained at circumcision in adult males with MGLSc (n = 4), paediatric LSc (n = 2) and normal healthy paediatric foreskin (n = 4). In adult samples 51 genes with significantly increased expression and 87 genes with significantly reduced expression were identified; paediatric samples revealed 190 genes with significantly increased expression and 148 genes with significantly reduced expression. Concordance of expression profiles between adult and paediatric samples indicates the same disease process. Functional analysis revealed increased expression in the adult and child MGSLc samples in the immune response/cellular defence gene ontology (GO) category and reduced expression in other categories including genes related to squamous cancer. No specific HPV, autoimmune or squamous carcinogenesis-associated gene expression patterns were found. ECM1 and CABLES1 expression were significantly reduced in paediatric and adult samples respectively.