Determination of 4,6-Dinitro-o-set-butylphenol Residues in Fruits and Almonds by Electron-Capture Gas Chromatography


  • The authors thank Dr. Joseph Ogawa, Department of Plant Pathology, for providing the field-treated samples.


SUMMARY— A procedure for the analysis of 4,6-dinitro-o-set-butylphenol (DNOSBP) in fruits and almonds by electron-capture gas chromatography is described. The alkanolamine salts of DNOSBP are converted to DNOSBP with acid prior to extraction with benzene. The extracted DNOSBP is then cleaned-up by column chromatography, methylated with diazomethane, and analyzed as the methyl ether. The overall average recovery of DNOSBP residues on almonds, cherries, peaches, and apricots (fortified with 0.01–0.5 ppm DNOSBP) was 90%.