OXIDATION OF MYOGLOBIN IN VITRO MEDIATED BY LIPID OXIDATION IN MICROSOMAL FRACTIONS OF MUSCLE

Authors


  • Paper No. 2026. Massachusetts Agricultural Experiment Station, University of Massichusetts at Amherst. This research was supported in part by Experiment Station Project No. 345.

ABSTRACT

Enzymic lipid peroxidation of a microsomal fraction prepared from chicken leg muscle led to the oxidation of oxymyoglobin to metmyoglobin when the former was incubated in vitro with the microsomal peroxidation system. Similar oxidation of pigment was observed in the presence of linolenate hydroperoxide. On prolonged incubation of myoglobin with the peroxidizing microsomal fraction, some destruction of the pigment occurred. Incubation with either BHA or a mixture of glutathione and glutathione peroxidase inhibited much of the pigment oxidation.

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