Presented at the 36th Annual Meeting of the Institute of Food Technologists, Anaheim, June 6–9, 1976.
GROWTH RESPONSE OF Bifidobacterium bifidum TO A HYDROLYTIC PRODUCT ISOLATED FROM BOVINE CASEIN
Article first published online: 25 AUG 2006
Journal of Food Science
Volume 42, Issue 1, pages 146–150, January 1977
How to Cite
KEHAGIAS, C., JAO, Y. C., MIKOLAJCIK, E. M. and HANSEN, P. M. T. (1977), GROWTH RESPONSE OF Bifidobacterium bifidum TO A HYDROLYTIC PRODUCT ISOLATED FROM BOVINE CASEIN. Journal of Food Science, 42: 146–150. doi: 10.1111/j.1365-2621.1977.tb01238.x
Journal Series Article No. 18.76 of the Ohio Agricultural Research and Development Center, Wooster.
Supported in part by Public Health Service Research Grants FD 117 and 462 from the Office of Research and Training Grants, FDA.
- Issue published online: 25 AUG 2006
- Article first published online: 25 AUG 2006
- MS received 4/28/76; revised 8/21/76; accepted 8/25/76.
In the process of isolating sialic acid from bovine isoelectric casein a hydrolytic product (CBF) was obtained, possessing growth promoting activity for Bifidobacterium bifidum var. pennsylvanicus. Growth was measured turbidimetrically by addition of CBF (250 ppm) to ATCC Medium 76. Stimulation by CBF was comparable to that obtained with N-acetylglucosamine. Whole casein, sialic acid-free casein and k-casein failed -to stimulate the growth of -the organism to the level achieved with CBF. The CBFfraction contained 0.15% nhosohorus. 7% free lactose and various peptides. No sialic acid or ofher amino sugars were detected. On the basis of phosphorus content and amino acid analysis it was concluded that CBF was likely a degradation product of k-casein.