The authors thank Pablo Cue for statistical services and Margaret V. Hall, Carol Johnson and Irene C. Smith for technical assistance.
QUANTITATIVE DETERMINATION OF CARRAGEENAN IN INFANT FORMULAS
Version of Record online: 25 AUG 2006
Journal of Food Science
Volume 42, Issue 1, pages 252–254, January 1977
How to Cite
KLECKNER, J., RICK, N. R. and THOMSON, W. A. B. (1977), QUANTITATIVE DETERMINATION OF CARRAGEENAN IN INFANT FORMULAS. Journal of Food Science, 42: 252–254. doi: 10.1111/j.1365-2621.1977.tb01263.x
- Issue online: 25 AUG 2006
- Version of Record online: 25 AUG 2006
- MS received 4/22/76; revised 7/27/76; accepted 7/30/76.
An accurate and precise method was developed for the determination of carrageenan in milk-based infant formulas. Following digestion of protein with papain, carrageenan was precipitated with Hyamine 1622, a quatemary ammonium salt. The precipitate was washed to remove lactose, dissolved in sulfuric acid and the carrageenan assayed by color development with the phenol-sulfuric reagent. Filtrations and washings were facilitated with Millipore filters. Carrageenan recovery of spiked-formulas averaged 99.67% for ready-to-feed products and 95.74% for concentrated products, which respectively contained 7.1% and 14.2% lactose. To obtain these accuracies the reference carrageenan must be identical to the carrageenan in the product.