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Bioconversion of Shellfish Chitin Waste: Waste Pretreatment, Enzyme Production, Process Design, and Economic Analysis
This research was sponsored in part by NOAA, National Sea Grant College Program, Dept. of Commerce, under Grant No. NA80AADIZOSEA Gr. Project No. R/F-34 and R/F-70, through the California Sea Grant College Program. The U.S. Government is authorized to produce and distribute reprints for governmental purposes notwithstanding any copyright notation that may appear hereon.
This research also received support from the Univ. of California Agricultural Experiment Station, under Project No. 3523-H.
The authors axe grateful to Canada Packers Limited (Toronto, Ontario) for supplying chitinase. The authors acknowledge the technical assistance of Manuel E. Young, Universidad de Chile; Sergio Revah, Universidad Autonoma Metropolitana-Iztapalapa, Mexico; Raymond A. Tom, Univ. of California at Davis; and David M. Ogrydziak, Univ. of California at Davis.
ABSTRACT
Study of pretreatment of shrimp processing waste for a chitin bioconversion scheme to produce yeast single-cell protein established conditions for size reduction, deproteination, and demineralization. Enzymatic hydrolysis of pretreated chitin waste achieved 80% conversion in 24 hr. Optimum temperature and pH were determined for maximum chitinase production in submerged culture, using pretreated chitin waste as substrate. An integrated process scheme for conversion of shrimp shell chitin waste to yeast single-cell protein based on these and previous results was designed and analyzed economically, giving a negative after-tax cash flow of $0.06 per kg of wet waste.