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ABSTRACT

Modified PA3679 agar (MPA3679A) was formulated as a plating medium to provide improved recovery of Clostridium sporogenes Putrefactive Anaerobe (PA) 3679 spores. A comparative study of MPA3679A and two currently used plating media, Yeast extract agar (YEA), and Peptone trypticase agar (PTA), showed that MPA3679A was superior for the recovery of heat activated and heat stressed spores. Further, when this medium was altered for use as an inoculated pack subculture medium, recovery of severely heat stressed spores was superior to that in the previously recommended PA3679 agar, also known as T-Best.