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ABSTRACT

An internally controlled gradient feed culturing technique, where a nutrient gradient was initiated and subsequently controlled by the lactic acid synthesis, was compared to conventional batch culturing for growth of a sausage starter organism, Lactobacillus plantarum. Significantly higher cell densities (p < 0.01) were observed with twice the culturing time for gradient feed culturing compared to batch culturing. Cell yields within batch or gradient feed culturing system were not affected by pH control at 5.8 or 6.0. Significantly higher (p < 0.05) cell yields, however, were obtained for gradient feed cultures (pH 6.0) with NH4OH addition at 50% theoretical lactic acid (TLA) level than were obtained at 10% TLA or without NH4OH addition.