A New Calculation Method for Distinguishing Endo- from Exo-Polygalacturonases



A method which uses a comparison ratio (CR) of fluidity (1/n)sp)/μmoles reducing groups is proposed for comparing and distinguishing endo- and exo-polygalacturonases (PGases). This method is faster, easier and as accurate a method as the percent hydrolysis method. The CR method can also be used as a measure of the purity of endo- PGase with respect to exo-PGase.