Combinations of sodium chloride and five different phosphates were used to study the effect of phosphate type (chain lengths: 1.0–12.8), ionic strength (0.15–0.43), and pH (5.50–6.35) on the binding ability (cook yield and tensile strength) of restructured beef rolls. The results showed that binding ability increased linearly with increasing ionic strength and pH until a maximum value was reached and then plateaued; approximately 80% of the increase in binding ability was due to the increase in ionic strength and pH. Above an ionic strength of 0.15, the polyphosphates (chain length > 1.0) produced synergistic increases in binding ability with increasing ionic strength. The extent of the synergistic effect decreased linearly as the chain length of the phosphate increased.