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ABSTRACT

The chlorogenic and caffeic acid combined in apple juice cultivars and ciders correlated very well with total phenol content (P leqslant R: less-than-or-eq, slant 0.001; r = 0.989; n = 22). The same correlation was found when analyzed strictly by cultivar (P leqslant R: less-than-or-eq, slant 0.001; r = 0.986; n = 10). Analysis used automated Folin Ciocalteu (FC) colorimetry values for total phenols and reversed-phase HPLC (280 nm detection) for chlorogenic and caffeic acid before and after fermentation. Concentrations were between 8 and 1012 mg/L (as chlorogenic acid) in different cultivars. The total chlorogenic acid by HPLC represented only 6.2 to 10.7% of the total polyphenol (calculated as chlorogenic acid) by FC in the apple cultivars for all samples. Total peak areas correlated very well with total phenol content by FC (P leqslant R: less-than-or-eq, slant 0.001; r = 0.994; n = 22).