Florida Agricultural Experiment Station Journal Series No. 9250.
Rapid Extraction of Lycopene and β-Carotene from Reconstituted Tomato Paste and Pink Grapefruit Homogenates
Article first published online: 25 AUG 2006
Journal of Food Science
Volume 55, Issue 5, pages 1460–1461, September 1990
How to Cite
SADLER, G., DAVIS, J. and DEZMAN, D. (1990), Rapid Extraction of Lycopene and β-Carotene from Reconstituted Tomato Paste and Pink Grapefruit Homogenates. Journal of Food Science, 55: 1460–1461. doi: 10.1111/j.1365-2621.1990.tb03958.x
- Issue published online: 25 AUG 2006
- Article first published online: 25 AUG 2006
- Ms received 3/9/90; revised 3/9/90; accepted 3/12/90.
Samples were extracted in Erlenmeyer flasks with hexane-ethanol-acetone (50:25:25). After extraction, 15 mL of water were added to separate the solution into polar and nonpolar layers. Lycopene was concentrated in the upper nonpolar phase while other components and polar pigments were contained in the lower polar phase. The lycopene phase was analyzed using isocratic HPLC with a C-18 column and methanol-THF-water (67:27:6) mobile phase. Lycopene and (β-caro-tene eluted as chromatographically pure peaks.