Contribution #90-286-J, Kansas Agricultural Experiment Station, Manhattan, KS 66506. This material is based upon work supported by the Cooperative State Research Service, U.S. Dept. of Agriculture, under Agreement No. 89-34187 4511.
Comparison of Rapid Methods for isolation and Enumeration of Clostridium perfringens in Meat
Article first published online: 25 AUG 2006
Journal of Food Science
Volume 56, Issue 2, pages 367–370, March 1991
How to Cite
ALI, M. S., FUNG, D. Y. C. and KASTNER, C. L. (1991), Comparison of Rapid Methods for isolation and Enumeration of Clostridium perfringens in Meat. Journal of Food Science, 56: 367–370. doi: 10.1111/j.1365-2621.1991.tb05282.x
- Issue published online: 25 AUG 2006
- Article first published online: 25 AUG 2006
- Ms received 5/25/90; revised 7/27/90; accepted 8/30/90.
Three strains of Clostridium pefringens, ATCC 12924, ATCC 12917, and ATCC 14809, were inoculated separately into thioglycollate broth and ground beef samples and enumerated by Fung's Double Tube (FDT), Oxyrase Enzyme (OE), Gas Pak Anaerobic System (GPAS), and Anaerobic Petri Dish (APD) methods. Recovery of the three strains by FDT was significantly higher (P < 0.05) than that by the other three methods from both thioglycollate broth and ground beef, with OE second highest. FDT gave better recovery (84%) in less time (8–10 hr) at 42°C compared with the OE method (61%). FDT was also least costly.