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ABSTRACT

The anthocyanins in the skin of lychee fruit were isolated and purified by Sephadex G-25 and LH-20 gel column chromatography, and Sep-Pak C-18 cartridge. Further separation by HPLC on a C-18 column, on-line spectra by photodiode array detection, and acid and alkali hydrolyses were used for identification. Cyanidin-3-rutinoside was the major anthocyanin found. Cyanidin-3-gIucoside and malvidin-3-ace-tylglucoside were also identified. Polymerized anthocyanin pigment was also present, appearing to contribute to the brownish-red color.