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ABSTRACT

The heat-stable fraction of covalently immobilized peroxidase on/in porous glass beads in an environment of dodecane was studied as a bioindicator for evaluation of thermal processes under conditions of pasteurization. The verified bioindicator had a z value of 10.1°C and a Dref (at 70°C) of 22 min. At processing temperatures of 70°C and 80°C, the lethalities (F)bio as read from the bioindicator agreed very well with the target Ft10.0 values calculated from the time temperature data according to the general method.