Short Thermal Treatment Effect on Carp Myofibril and Sarcoplasmic Reticulum: Possible Mechanisms in Rigor Mortis Acceleration by “Arai” Treatment

Authors

  • SHUGO WATABE,

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • GYU-CHUL HWANG,

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • HIDEKI USHIO,

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • HIDEAKI YAMANAKA,

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • KEIKO HATAE,

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • KANEHISA HASHIMOTO

    1. Authors Watabe, Hwang, Ushio and Hashimoto are with the Laboratory of Marine Biochemistry, Faculty of Agriculture, Univ. of Tokyo, Bunkyo, Tokyo 113, Japan. Author Yamanaka is with the Dept. of Food Science & Technology, Tokyo Univ. of Fisheries, Minato, Tokyo 108, Japan. Author Hatae is with the Dept. of Food & Nutrition. Ochanomizu Univ., Bunkyo, Tokyo 112, Japan. Author Hashimoto's present address: Food Science Laboratory, Faculty of Education, Ibaraki Univ., Mito, Ibaraki 310, Japan.
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  • We express heartfelt thanks to Dr. Akihiro Okitani, Nippon Veterinary & Zootechnical College, for invaluable advice during the course of this study.

  • Costa of this study were partly defrayed by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

ABSTRACT

Inorganic γ-phosphate liberation from adenosine 5′-triphosphate (ATP) by carp myofibrillar ATPase was measured at 0-60°C to elucidate mechanisms in rigor mortis acceleration of sliced carp muscle during washing at a moderately high temperature. ATP splitting within 20 sec in the presence of 5 mM MgCl2 plus 0.25 mM CaCl2 was maximal at 45°C, which agreed well with the commercially adopted condition for preparing carp “arai” muscle. In addition, the maximum Ca2+ uptake by sarcoplasmic reticulum was observed at 30°C and decreased at higher temperatures. The acceleration of carp muscle rigor mortis at around 45°C was suggested to be partly due to enhancement of myofibrillar Mg2+-ATPase activity by increase of intracellular Ca2+ concentration.

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