Aflatoxin and Cyclopiazonic Acid Production by Aspergillus flavus Isolated from Contaminated Maize

Authors

  • Y. JENNIFER LEE,

    1. Authors Lee and Hagler are with the Mycotoxin Laboratory, Dept. of Poultry Science, North Carolina State Univ., 1320 Varsity Drive, Raleigh, NC 27695-7636. Address inquiries to Dr. Hagler.
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  • WINSTON M. HAGLER JR.

    1. Authors Lee and Hagler are with the Mycotoxin Laboratory, Dept. of Poultry Science, North Carolina State Univ., 1320 Varsity Drive, Raleigh, NC 27695-7636. Address inquiries to Dr. Hagler.
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  • Technical assistance of Faye Suggs and T. Still is appreciated. Use of trade names does not imply endorsement by the North Carolina Agricultural Research Service nor disapproval of similar products not mentioned.

ABSTRACT

Seven truck-loads of maize were tested for mycotoxin contamination. Aflatoxin was identified in all 7 at concentrations from 3 ng/g-501 ng/g (aflatoxin B1+ B2). Cyclopiazonic acid was identified in 4 loads with concentrations from 25-250 ng/g. Deoxynivalenol was found in 4 of 5 loads tested, over a range of 46-676 ng/g. Ninteeen isolates of Aspergillus flavus from the samples were tested for ability to accumulate cyclopiazonic acid and aflatoxin in liquid culture. Fourteen produced cyclopiazonic acid (0.5-135 μg/mL), 12 produced aflatoxin (0.01-0.70 μg/mL, aflatoxin B1+ B2), and one aflatoxin-producing isolate did not produce cyclopiazonic acid.

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