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Keywords:

  • microbes;
  • liquid media;
  • growth;
  • spoilage;
  • B. thermosphacta;
  • P. fluorescens

ABSTRACT

Comparisons of linear, square root and Arrhenius specific growth rate-temperature models showed no clear overall preference. At low aw and refrigeration temperature, the linear model was satisfactory for Brochothrix thermosphacta and Pseudomonas fluorescens. A linear relationship between (specific growth rate)−1 and lag phase was observed for these two psychrotrophs. The R2 for B. thermosphacta in 3% (aw= 0.968), 6% (aw= 0.950), and 9% NaCl (aw= 0.943) media was 0.865, 0.946 and 0.994, respectively. In 8% (aw= 0.973) and 20% (aw= 0.948) glycerol media, the R2 was 0.998 and 0.999, respectively. R2 for P. fluorescens growth in NaCl (3 or 4%) or glycerol (10 or 20%) media was >0.99. A cumulative growth adaptation function successfully estimated microbial growth at fluctuating temperatures.