Microbial Growth Estimation in Liquid Media Exposed to Temperature Fluctuations
Article first published online: 26 AUG 2006
Journal of Food Science
Volume 58, Issue 3, pages 644–648, May 1993
How to Cite
LI, K.-Y. and TORRES, J. A. (1993), Microbial Growth Estimation in Liquid Media Exposed to Temperature Fluctuations. Journal of Food Science, 58: 644–648. doi: 10.1111/j.1365-2621.1993.tb04347.x
- Issue published online: 26 AUG 2006
- Article first published online: 26 AUG 2006
- MS received 11/16/92; revised l/8/93; accepted l/24/93.
- liquid media;
- B. thermosphacta;
- P. fluorescens
Comparisons of linear, square root and Arrhenius specific growth rate-temperature models showed no clear overall preference. At low aw and refrigeration temperature, the linear model was satisfactory for Brochothrix thermosphacta and Pseudomonas fluorescens. A linear relationship between (specific growth rate)−1 and lag phase was observed for these two psychrotrophs. The R2 for B. thermosphacta in 3% (aw= 0.968), 6% (aw= 0.950), and 9% NaCl (aw= 0.943) media was 0.865, 0.946 and 0.994, respectively. In 8% (aw= 0.973) and 20% (aw= 0.948) glycerol media, the R2 was 0.998 and 0.999, respectively. R2 for P. fluorescens growth in NaCl (3 or 4%) or glycerol (10 or 20%) media was >0.99. A cumulative growth adaptation function successfully estimated microbial growth at fluctuating temperatures.