Novel Measurement of Hypoxanthine in Fish Using Direct Measurement Probe and Chemiluminescence Flow Injection Analysis

Authors

  • KENJI HAYASHI,

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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  • TAKUJI OKUGAWA,

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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  • YOSHIAKI KOZUKA,

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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  • SATOSHI SASAKI,

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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  • KAZUNORI IKEBUKURO,

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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  • ISAO KARUBE

    1. Authors Hayashi, Okugawa, and Kozuka are with Nichirei Corporation, 6–19–20 Tsukiji, Chuo-ku, Tokyo 104, Japan. Authors Sasaki, Ikebukuro, and Karube are with the Research Center for Advanced Science & Technology, Univ. of Tokyo, 4–6–1 Komaba, Meguro-ku, Tokyo 153, Japan. Direct inquiries to Dr. Isao Karube.
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ABSTRACT

Direct measurements of hypoxanthine (Hx) in fish flesh were carried out using a sardine and a grunt twice with the same fish, and the average of 10 successive measurements (from 120 sec to 480 sec after pressing the probe against fish tissue) was taken as the Hx concentration by direct measurement. Results with two nearby tissues of one sample were 42.9 ± 4.9 μM and 42.6 ± 2.6 μM for the sardine, and 639 ± 33 μM and 598 ± 29 μM for the grunt. For sardine, Hx results from this direct method did not agree well with perchloric acid extraction measurements, but they were much better for grunt.

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