This work was supported by research grants from Formation de Chercheurs et L'aide a la Recherche (FCAR) and Natural Science and Engineering Research Council (NSERC) of Canada.
Control of Endogenous Enzyme Activity in Fish Muscle by Inhibitors and Hydrostatic Pressure using RSM
Article first published online: 26 AUG 2006
Journal of Food Science
Volume 61, Issue 2, pages 350–356, March 1996
How to Cite
ASHIE, I.N.A., SIMPSON, B.K. and RAMASWAMY, H.S. (1996), Control of Endogenous Enzyme Activity in Fish Muscle by Inhibitors and Hydrostatic Pressure using RSM. Journal of Food Science, 61: 350–356. doi: 10.1111/j.1365-2621.1996.tb14192.x
- Issue published online: 26 AUG 2006
- Article first published online: 26 AUG 2006
- Ms received 6/8/95; revised 10/20/95; accepted 11/10/95.
- hydrostatic pressure, fish proteases;
- response surface
Effects of combination treatments involving hydrostatic pressure, pH, and crude α;2-macroglobulin on muscle protease activity and viscosity were investigated using response surface methodology. Statistical analysis showed the model was very significant, predicting responses with high accuracy. Proteolytic activities were reduced with increasing pressure and inhibitor concentrations while pH elicited a quadratic response. Reducing pH and increasing pressure also reduced viscosity of tissue homogenate, but inhibitor concentration did not affect (p > 0.05) this response. The proteases did not show any tendency to regain activity during 3 weeks storage possibly due to a crude inhibitor in the homogenate and did not seem to have any direct influence on viscosity as shown by a low correlation (r =–0.30). Viscosity progressively increased during this period.