Fluorometric Assay for Screening Antioxidative Activity of Vegetables

Authors

  • S. FURUTA,

    1. Authors Furuta, Nishiba, and Suda are affiliated with Kyushu National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Nishigoshi, Kumamoto 861-11, Japan.
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  • Y. NISHIBA,

    1. Authors Furuta, Nishiba, and Suda are affiliated with Kyushu National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Nishigoshi, Kumamoto 861-11, Japan.
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  • I. SUDA

    1. Authors Furuta, Nishiba, and Suda are affiliated with Kyushu National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, Nishigoshi, Kumamoto 861-11, Japan.
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  • We thank Ms. M. Yoshida for technical assistance.

ABSTRACT

A fluorometric assay for evaluating antioxidative activities was principally based on inhibition of lipid peroxidation accompanied by the autoxidation of linoleic acid. It could estimate the antioxidative activities of vegetable extracts as well as known antioxidants in a linear response. All analytical procedures could be performed within a few hours using one test tube. The assay enabled testing a large number of samples treated at one time and application to vegetables containing pigments.

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