Oxymyoglobin Oxidation as Affected by Oxidation Products of Phosphatidylcholine Liposomes

Authors

  • WENDY K.M. CHAN,

    1. Authors Chan and Faustman are with the Dept. of Animal Science, Univ. of Connecticut, Storrs, CT 06269. Author Decker is with the Dept. of Food Science, Univ. of Massachusetts, Amherst, MA 01003. Direct inquiries to Dr. W.K.M. Chan.
    Search for more papers by this author
  • CAMERON FAUSTMAN,

    1. Authors Chan and Faustman are with the Dept. of Animal Science, Univ. of Connecticut, Storrs, CT 06269. Author Decker is with the Dept. of Food Science, Univ. of Massachusetts, Amherst, MA 01003. Direct inquiries to Dr. W.K.M. Chan.
    Search for more papers by this author
  • ERIC A. DECKER

    1. Authors Chan and Faustman are with the Dept. of Animal Science, Univ. of Connecticut, Storrs, CT 06269. Author Decker is with the Dept. of Food Science, Univ. of Massachusetts, Amherst, MA 01003. Direct inquiries to Dr. W.K.M. Chan.
    Search for more papers by this author

  • Support was provided by the U.S. Army Natick RD&E Center, USDA NRICGP, the Storrs Agricultural Experiment Station, College of Agriculture & Natural Resources, the Univ. of Connecticut Research Foundation, Univ. of Connecticut, and Hoffmann-LaRoche Inc. Scientific contribution number 1741, Storrs Agricultural Experiment Station.

ABSTRACT

Supernatants (105,000 ×g) of oxidized phosphatidylcholine (PC) liposomes contained higher levels of thiobarbituric acid-reactive substances, and caused greater metmyoglobin formation when combined with oxymyoglobin than those of controls (C) or PC plus tocopherol (PCT) (P<0.05). Products of PC liposome oxidation crossed dialysis membranes (mol wt cut-off = 500 Dalton) and accelerated the oxidation of oxymyoglobin more than C or PCT (P<0.05). The addition of known oxidation products of oleic acid and linoleic acid, fatty acids typical of PC, accelerated oxymyoglobin oxidation relative to controls (P<0.05). Results demonstrated that oxidation products of PC were prooxidative towards oxymyoglobin.

Ancillary