This work was supported by a grant No ALI95–0306 from the Comisión Interministerial de Ciencia y Tecnología (CICYT) of Spain. A. Céspedes is a recipient of a fellowship from the Comunidad Autónoma de Castilla-La Mancha; E. Carrera and I. González are recipients of a fellowship from the Ministerio de Educación y Ciencia (Spain). The authors thank Angel Mendizabal (S.V.O., Mercamadrid) for kindly supplying fish samples.
Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene
Version of Record online: 28 JUN 2008
Journal of Food Science
Volume 63, Issue 2, pages 206–209, March 1998
How to Cite
CÉSPEDES, A., GARCÍA, T., CARRERA, E., GONZÁLEZ, I., SANZ, B., HERNÁZ, P. E. and MARTÍN, R. (1998), Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene. Journal of Food Science, 63: 206–209. doi: 10.1111/j.1365-2621.1998.tb15710.x
- Issue online: 28 JUN 2008
- Version of Record online: 28 JUN 2008
- Ms received 6/8/97; revised 10/8/97; accepted 10/11/97.
- cytochrome b
Restriction site analysis of PCR products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa) and flounder (Platichthys flesus). Polymerase chain reaction (PCR) amplification of the cytochrome b gene using universal primers produced a 359 bp fragment in all species analyzed. Digestion of the PCR products with Nci I, Sau 3AI and Hinf I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of the fish species. This methodology should prove useful for enforcing labeling regulations in the authentication of flatfish species.