The authors gratefully acknowledge financial support provided by the Univ. of Wisconsin-Madison, College of Agricultural & Life Sciences.
Development of a Simple Method for Detecting Presumptive Escherichia coli on Fresh Retail Beef
Article first published online: 20 JUL 2006
Journal of Food Science
Volume 67, Issue 1, pages 258–261, January 2002
How to Cite
Bolling, B.W., Schmidt, D.J. and Ingham, S.C. (2002), Development of a Simple Method for Detecting Presumptive Escherichia coli on Fresh Retail Beef. Journal of Food Science, 67: 258–261. doi: 10.1111/j.1365-2621.2002.tb11394.x
- Issue published online: 20 JUL 2006
- Article first published online: 20 JUL 2006
- MS 20000620, Submitted 6/7/2000, Accepted 6/7/2001
- E. coli detection;
- retail beef
ABSTRACT: A simple method of detecting Escherichia coli on retail beef was developed. Diluent used in sponge-sampling of meat was added to a tube containing double-strength lauryl sulfate tryptone broth + 5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-GlcA) and a Durham tube. Tubes with blue color and gas after 24 h incubation at 35 °C were presumptive-positive. Chi-squared analysis showed a significant (P < 0.05) relationship between the method and the Petrifilm™E. coli/Coliform Count Plate method for E. coli in analysis of 66 retail beef samples and the 2 methods had a similar proportion of confirmed isolates (61 and 63%). The method developed is qualitatively comparable to the Petrifilm™ method and provides small-scale beef processors a simple way to monitor sanitary conditions in fabrication and grinding operations.