• E. coli detection;
  • retail beef

ABSTRACT: A simple method of detecting Escherichia coli on retail beef was developed. Diluent used in sponge-sampling of meat was added to a tube containing double-strength lauryl sulfate tryptone broth + 5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-GlcA) and a Durham tube. Tubes with blue color and gas after 24 h incubation at 35 °C were presumptive-positive. Chi-squared analysis showed a significant (P < 0.05) relationship between the method and the Petrifilm™E. coli/Coliform Count Plate method for E. coli in analysis of 66 retail beef samples and the 2 methods had a similar proportion of confirmed isolates (61 and 63%). The method developed is qualitatively comparable to the Petrifilm™ method and provides small-scale beef processors a simple way to monitor sanitary conditions in fabrication and grinding operations.