The authors acknowledge the financial support by the National Oceanic and Atmospheric I Administration, U.S. Dept. of Commerce, Washington, D.C., through Grants No. NA46RG013704 to the National Coastal Resources Research and Development Institute, Portland, Oreg.
Development of Biogenic Amines in Yellowfin Tuna (Thunnus albacares): Effect of Storage and Correlation with Decarboxylase-Positive Bacterial Flora
Article first published online: 20 JUL 2006
Journal of Food Science
Volume 67, Issue 1, pages 292–301, January 2002
How to Cite
Du, W.-X., Lin, C.-M., Phu, A.-T., Cornell, J.A., Marshall, M.R. and Wei, C.-I. (2002), Development of Biogenic Amines in Yellowfin Tuna (Thunnus albacares): Effect of Storage and Correlation with Decarboxylase-Positive Bacterial Flora. Journal of Food Science, 67: 292–301. doi: 10.1111/j.1365-2621.2002.tb11400.x
- Issue published online: 20 JUL 2006
- Article first published online: 20 JUL 2006
- MS 20001543, Submitted 10/30/00, Revised 7/18/01, Accepted 7/24/01, Received 8/8/01
- biogenic amines;
- fluorometric analysis;
- capillary electrophoresis
ABSTRACT: The effects of storage at 0,4,10, and 22°C for 0,1,3,5, and 9 d on the quality of yellowfin tuna fillets as determined by microbiological assessment, development of some biogenic amines, and sensory analysis were studied. Tuna fillets stored at 22 °C for 3 d, 10 °C for 5 d, and 4 °C for 9 d were rated unacceptable for consumption. Those stored at 22 °C for 3 d had total aerobic bacterial count of > 8 log10 CFU/g, a histamine-producing bacterial population of 7 log10 CFU/g, and 832 ppm of histamine, 35.8 ppm of putrescine, and 147 ppm of cadaverine. A comparison of the capillary electrophoresis, AOAC fluorometric method, and gas chromatography showed a very good correlation (r2 > 0.99) among these 3 methods for histamine quantitation in tuna samples. Morganella morganii, Enterobacter agglomerans, Enterobacter intermedium, Pseudomonas fluorescens, Proteins vulgaris, and Serratia liquefaciens were the decarboxylase-positive bacterial species isolated by using the Niven's medium and identified during storage, which were responsible for histamine production in test tuna fillets.