Piezoelectric Flow Injection Analysis Biosensor for the Detection of Salmonella Typhimurium

Authors

  • S. Babacan,

    1. The authors are with the Fiber Optic and Biosensor Research Group of the Sensor and Surface Technology Partnership, at Univ. of Rhode Island, U.S.A. Authors Babacan and Rand are with the Food Science and Nutrition Dept., 530 Liberty Lane, West Kingston, RI02892. Authors Pivarnik and Letcher are with the Physics Dept., Univ. of Rhode Island, Kingston, RI 02881. Direct inquiries to author Rand (E-mail: ara8126@postoffice.uri.edu).
    Search for more papers by this author
  • P. Pivarnik,

    1. The authors are with the Fiber Optic and Biosensor Research Group of the Sensor and Surface Technology Partnership, at Univ. of Rhode Island, U.S.A. Authors Babacan and Rand are with the Food Science and Nutrition Dept., 530 Liberty Lane, West Kingston, RI02892. Authors Pivarnik and Letcher are with the Physics Dept., Univ. of Rhode Island, Kingston, RI 02881. Direct inquiries to author Rand (E-mail: ara8126@postoffice.uri.edu).
    Search for more papers by this author
  • S. Letcher,

    1. The authors are with the Fiber Optic and Biosensor Research Group of the Sensor and Surface Technology Partnership, at Univ. of Rhode Island, U.S.A. Authors Babacan and Rand are with the Food Science and Nutrition Dept., 530 Liberty Lane, West Kingston, RI02892. Authors Pivarnik and Letcher are with the Physics Dept., Univ. of Rhode Island, Kingston, RI 02881. Direct inquiries to author Rand (E-mail: ara8126@postoffice.uri.edu).
    Search for more papers by this author
  • A. Rand

    1. The authors are with the Fiber Optic and Biosensor Research Group of the Sensor and Surface Technology Partnership, at Univ. of Rhode Island, U.S.A. Authors Babacan and Rand are with the Food Science and Nutrition Dept., 530 Liberty Lane, West Kingston, RI02892. Authors Pivarnik and Letcher are with the Physics Dept., Univ. of Rhode Island, Kingston, RI 02881. Direct inquiries to author Rand (E-mail: ara8126@postoffice.uri.edu).
    Search for more papers by this author

  • Contribution Nr 3845 of the Rhode Island Agricultural Experimental Station. This study was sponsored by grants to URI Fiber Optic and Biosensor Research Group by the Cooperative State Research, Education, and Extension Service, U.S. Dept. of Agriculture under NRICGP Agreement Nr 93-37201-9197, andby the URI Sensor and Surface Technology Partnership. We thankLewis Shrady and David Butler for their input on the piezoelectric system, and Dr. Mike Platek for his assistance with AFM.

Abstract

ABSTRACT: Piezoelectric biosensors have the potential to provide direct detection of food contaminants, such as pathogens. In this study, Protein A antibody immobilization was used for the activation of the piezoelectric biosensor to detect Salmonella typhimurium. The overall system consisted of a new design for a flow cell and flow injection analysis system. The flow cell made possible a baseline stability of ± 1 Hz out of 5 MHz for hours. The sensor had responses of 5 to 65 Hz in 30 min with R2= 0.95 for S. typhimurium concentrations of 107to 109 CFU/ml under continuous flow, and 3 to 75 Hz in 40 min with R2= 0.96 for S. typhimurium concentrations of 106 to 1010 CFU/ ml under stop flow. Cross-reactivity tests were performed with nonpathogenic Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, and Vibrio parahaemolyticus and showed less than 10% response.

Ancillary