Chemopreventive Potential of Wild Lowbush Blueberry Fruits in Multiple Stages of Carcinogenesis

Authors

  • Tristan F. Burns Kraft,

    1. Authors Kraft and Lila are with Div. of Nutritional Sciences, Univ. of Illinois at Ur-bana-Champaign, 1201 S. Dorner Dr., Urbana, IL 61801.
    2. Authors Kraft, Schmidt, Yousef, Knight, and Lila are with Dept. of Natural Resources and Environmental Sciences, Univ. of Illinois at Urbana-Champaign, Urbana, Ill.
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  • Barbara M. Schmidt,

    1. Authors Kraft, Schmidt, Yousef, Knight, and Lila are with Dept. of Natural Resources and Environmental Sciences, Univ. of Illinois at Urbana-Champaign, Urbana, Ill.
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  • G.G. Yousef,

    1. Authors Kraft, Schmidt, Yousef, Knight, and Lila are with Dept. of Natural Resources and Environmental Sciences, Univ. of Illinois at Urbana-Champaign, Urbana, Ill.
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  • Christopher T.G. Knight,

    1. Authors Kraft, Schmidt, Yousef, Knight, and Lila are with Dept. of Natural Resources and Environmental Sciences, Univ. of Illinois at Urbana-Champaign, Urbana, Ill.
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  • Muriel Cuendet,

    1. Authors Cuendet and Kang are with Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago, Chicago, Ill.
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  • Young-Hwa Kang,

    1. Authors Cuendet and Kang are with Dept. of Medicinal Chemistry and Pharmacognosy, Univ. of Illinois at Chicago, Chicago, Ill.
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  • John M. Pezzuto,

    1. Author Pezzuto is with School ofPharmacy, Purdue Univ., West Lafayette, Ind.
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  • David S. Seigler,

    1. Author Seigler is with Department of Plant Biology, Univ. of Illinois at Urbana-Champaign, Urbana, Ill. Direct inquiries to author Lila (E-mail: imagemal@uiuc.edu).
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  • Mary Ann Lila

    1. Authors Kraft and Lila are with Div. of Nutritional Sciences, Univ. of Illinois at Ur-bana-Champaign, 1201 S. Dorner Dr., Urbana, IL 61801.
    2. Authors Kraft, Schmidt, Yousef, Knight, and Lila are with Dept. of Natural Resources and Environmental Sciences, Univ. of Illinois at Urbana-Champaign, Urbana, Ill.
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Abstract

ABSTRACT: Wild lowbush blueberry fruit extract was fractionated using vacuum chromatography and analyzed for chemopreventive potential using bioassays that test the ability of compounds to inhibit the initiation, promotion, and progression stages of carcinogenesis. A fraction containing phytosterols was active against the initiation stage (quinone reductase assay). However, more polar compounds were inhibitors of later stages of carcinogenesis; a fraction containing flavan-3-ols and fractions containing mainly anthocyanins, phenolic acids, flavan-3-ols, and some proanthocyanidin dimers demonstrated activity against the promotion stage (cyclooxygenase and ornithine decarboxylase assays, respectively), and a proanthocyanidin-rich fraction demonstrated antiproliferation activity (inhibition of cancerous murine hepatocyte proliferation is associated with the progression stage). These results indicate that lowbush blueberries contain a range of compounds that have bioactivity against multiple stages of carcinogenesis, and different types of phenolic compounds are active at different stages.

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