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Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Authors

  • Zhanhui Wang,

    1.  Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
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  • Suxia Zhang,

    1.  Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
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  • Nailya R. Murtazina,

    1.  Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119998, Russia
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  • Sergei A. Eremin,

    1.  Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow 119998, Russia
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  • Jianzhong Shen

    Corresponding author
    1.  Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
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*Fax: +86 10 62731032;
e-mail: sjz@cau.edu.cn

Summary

A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein-labelled MD (tracer) was synthesised by N-hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 μg mL−1 with an IC50 value of 0.16 μg mL−1 and a limit of detection of 0.002 μg mL−1. Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 μg g−1 levels were 82–130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable.

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