Seasonal Expression of 2 Types of Myosin with Different Thermostability in Silver Carp Muscle (Hypophthalmichthys molitrix)
Article first published online: 31 MAY 2006
Journal of Food Science
Volume 71, Issue 1, pages C39–C43, January 2006
How to Cite
Yuan, C., Kaneniwa, M., Wang, X., Chen, S., Cheng, Y., Qu, Y., FUKUDA, Y. and Konno, K. (2006), Seasonal Expression of 2 Types of Myosin with Different Thermostability in Silver Carp Muscle (Hypophthalmichthys molitrix). Journal of Food Science, 71: C39–C43. doi: 10.1111/j.1365-2621.2006.tb12386.x
- Issue published online: 31 MAY 2006
- Article first published online: 31 MAY 2006
- MS 20050389 Submitted 7/1/05, Revised 8/15/05, Accepted 10/18/05.
- silver carp;
- Ca-ATPase activity;
Myofibrils were prepared from dorsal muscle of silver carp monthly from Jul 2003 to Jun 2004. Myofibrils of silver carp in the summer season were much more stable than those in the winter season as measured by (Ca-ATPase) thermal inactivation rate (kD) at 0.1 M KCl. The kD of myofibrils measured at 2 M KCl was referred as 1 for myosin without protection by F-actin. It was proved that myosin itself in summer season fish (summer-type) was very stable compared with that in winter season fish (winter-type). Winter-type myosin easily denatured upon storage in ice. Analysis of the thermal inactivation profiles of myosin monthly prepared demonstrated that myosins in December, January, February, and March were winter-type, and ones in June, July, August, and September were summer-type. It was also demonstrated that both winter-type and summer-type myosin at different ratios coexisted in the transit season (April, May, October, November), showing the breaking point in the inactivation profile. It was concluded that silver carp expresses 2 types of myosin with different kD seasonally and that the exchange of the expression completed in 2 mo.