Absorption-enhancing Treatments for Antihypertensive Activity of Oligopeptides from Tuna Cooking Juice: In Vivo Evaluation in Spontaneously Hypertensive Rats

Authors

  • Wen-Ching Ko,

    1. Authors Ko is with Dept. of Bioindustry Technology, Da-Tsuen Univ., Changhua, Taiwan. Author Cheng is with Dept. of Nursing, Natl. Taichung Nursing College, Taichung, Taiwan. Author Hsu is with Dept. of Healthy Diet and Restaurant Management, Chung Shan Medical Univ., Taichung, Taiwan. Author Hwang is with Dept. of Food and Nutrition, Hung Kuang Univ., 34 Chung-Chie Rd., Sha-Lu, Taichung 433, Taiwan. Direct inquiries to author Hwang (E-mail: jyh3478@ms64.url.com.tw).
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  • Mei-Li Cheng,

    1. Authors Ko is with Dept. of Bioindustry Technology, Da-Tsuen Univ., Changhua, Taiwan. Author Cheng is with Dept. of Nursing, Natl. Taichung Nursing College, Taichung, Taiwan. Author Hsu is with Dept. of Healthy Diet and Restaurant Management, Chung Shan Medical Univ., Taichung, Taiwan. Author Hwang is with Dept. of Food and Nutrition, Hung Kuang Univ., 34 Chung-Chie Rd., Sha-Lu, Taichung 433, Taiwan. Direct inquiries to author Hwang (E-mail: jyh3478@ms64.url.com.tw).
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  • Kuo-Chiang Hsu,

    1. Authors Ko is with Dept. of Bioindustry Technology, Da-Tsuen Univ., Changhua, Taiwan. Author Cheng is with Dept. of Nursing, Natl. Taichung Nursing College, Taichung, Taiwan. Author Hsu is with Dept. of Healthy Diet and Restaurant Management, Chung Shan Medical Univ., Taichung, Taiwan. Author Hwang is with Dept. of Food and Nutrition, Hung Kuang Univ., 34 Chung-Chie Rd., Sha-Lu, Taichung 433, Taiwan. Direct inquiries to author Hwang (E-mail: jyh3478@ms64.url.com.tw).
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  • Jyh-Sheng Hwang

    1. Authors Ko is with Dept. of Bioindustry Technology, Da-Tsuen Univ., Changhua, Taiwan. Author Cheng is with Dept. of Nursing, Natl. Taichung Nursing College, Taichung, Taiwan. Author Hsu is with Dept. of Healthy Diet and Restaurant Management, Chung Shan Medical Univ., Taichung, Taiwan. Author Hwang is with Dept. of Food and Nutrition, Hung Kuang Univ., 34 Chung-Chie Rd., Sha-Lu, Taichung 433, Taiwan. Direct inquiries to author Hwang (E-mail: jyh3478@ms64.url.com.tw).
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Abstract

ABSTRACT The aim of this study was to evaluate the absorption-enhancing effect for antihypertensive activity of an angiotensin I-converting enzyme (ACE) inhibitory oligopeptides in spontaneously hypertensive rats (SHR). The oligopeptides OA3, derived from tuna cooking juice, significantly reduced systolic blood pressure (SBP) in SHR at the doses of 0.5, 0.75, and 1.0 g/kg body weight; their efficacies were exhibited in a dose-dependent manner. Emulsification, microencapsulation, and lipophilization were applied to enhance the antihyperten-sive activity of OA3 at the dose of 0.5 g/kg body weight in SHR individually. Lipophilization revealed most effectively by 16 mm Hg in SBP in SHR 4 h after oral administration; the significant different effect lasted more than 6 h. Such efficacy is greater than that from no further treated OA3 with the dose of 1.0 g/kg body weight. Enhancing treatments by using 30% gum Arabic and 30% lecithin exhibited minor effects by 13 and 11 mm Hg, respectively. Results imply that these additional treatments could further enhance and extend the antihyperten-sive effect of OA3 oligopeptides. This suggests that absorption-enhancing treatments improve the bioavailability of oligopeptides to exhibit higher antihypertensive effect. Furthermore, these enhancing effects may be brought about by the changes in cell membrane permeation or the protection of oligopeptides.

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