ABSTRACT: A new, all aqueous high-performance liquid chromatography (HPLC) procedure for the determination of 5-hydroxymethylfurfural (HMF) in honey and other sugar-containing materials was developed without harmful chemicals. The method involved dilution of the sample in water and the analysis of the diluent using an HPLC system equipped with an absorbance detector and a 30-mm sulfonated poly(styrene-divinylbenzene) cation exchange column. Water was used as the mobile phase at a high flow rate of 2.00 mL/min Using this high flow rate in conjunction with a column heater set at 55°C, HMF eluted in less than 4 min. Overall precision was less than 1% relative standard deviation, recovery of HMF from spiked samples was approximately 99%, and there were no interferences from similar compounds such as 2-furfural and 5-methylfurfural. Several sugar-containing materials were analyzed by this method where the results compared well with the classic Winkler spectrophotometric procedure, but not as well with a commonly used reverse-phase HPLC technique. In addition, several consumer products were also analyzed for their HMF content.