We thank Dr. Nikaido at the Tokyo Inst. of Technology and Ms. Nojima at Central Customs Laboratory for specific comments on this manuscript, Dr. Yamashita and Mr. Takashima at the Natl. Research Inst. of Fisheries Science and Dr. Saruwatari at Ocean Research Inst., The Univ. of Tokyo, for providing information, Dr. Endo at Kochi Univ. for identifying several standard fishes and Customs officers for assisting with our research.
Species Identification and PCR-RFLP Analysis of Cytochrome b Gene in Cod Fish (Order Gadiformes) Products
Article first published online: 30 JUN 2006
Journal of Food Science
Volume 71, Issue 3, pages C190–C195, April 2006
How to Cite
Akasaki, T., Yanagimoto, T., Yamakami, K., Tomonaga, H. and Sato, S. (2006), Species Identification and PCR-RFLP Analysis of Cytochrome b Gene in Cod Fish (Order Gadiformes) Products. Journal of Food Science, 71: C190–C195. doi: 10.1111/j.1365-2621.2006.tb15616.x
- Issue published online: 30 JUN 2006
- Article first published online: 30 JUN 2006
- MS 20050629 Submitted 10/20/05, Revised 12/30/05, Accepted 1/30/06.
- cod fish products;
- species identification;
- phylogenetic analysis
ABSTRACT: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) profiles using the PCR product tRNAGlu-cytochrome b of 9 standard cod fish, all of which are imported into Japan under the name “cod fish,” were investigated as a means of rapid species identification of imported cod fish products. Endonuclease digestion using 4 restriction enzymes (Alu I, Fok I, MboI, and Mse I) generated different digestion patterns for the standards, enabling species identification in imported cod fish products by comparison to the standards’RFLP profiles. Additionally, we conducted a more strict check by phylogenetic analysis on imported cod fish products with 13 standards (including 4 additional cod species from GenBank) using the mitochondrial cytochrome b gene (385 nt). Consequently, it was found that the PCR-RFLP method was sufficient for rapidly screening cod products, and in cases that require conclusive identification, phylogenetic analysis was the most suitable.