Effects of Reducing Agent Concentration on Soy Protein Fractionation and Functionality

Authors


  • This journal article of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, project nr 6571, was supported in part by a USDA Natl. Research Initiative (grant nr 2001-35503-10814), the Center for Crops Utilization Research, and Hatch Act and State of Iowa funds.

Abstract

ABSTRACT: The concentration of the reducing agent SO2 significantly affected fraction yields, purities, and compositions during soy protein fractionation, especially the purity of the glycinin-rich fraction. The optimum amount of reducing agent was 5 mM SO2 based on protein yields, purities, and functional properties. With no SO2, the glycinin-rich fraction contained 29% of the total protein with only 63% glycinin, and the β-conglycinin-rich fraction contained 10% of the total protein with 94%β-conglycinin; whereas, by adding 5 mM SO2, the glycinin-rich fraction contained 23.4% of total protein with 81.5% glycinin, and the β-conglycinin-rich fraction contained 16.8% of the total protein with 83.7%β-conglycinin. Increasing amounts of storage proteins were lost in the whey fraction as SO2 concentration increased. The functional properties of the 2 major fractions were greatly influenced by the addition of SO2. The thermal behaviors of the fractions were only slightly affected by using higher amounts of SO2. The solubility and hydrophobicity of the glycinin-rich fraction decreased with increasing SO2 concentration, whereas the solubility of the β-conglycinin-rich fraction increased. Emulsification properties of the glycinin-rich fraction were adversely affected by higher SO2 concentrations, whereas, those of the β-conglycinin-rich fraction were improved. The β-conglycinin-rich fractions had better emulsification properties than did the glycinin-rich fractions. The best foaming properties were achieved at 5 mM SO2.

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