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Optimisation of the microwave-assisted extraction process for six phenolic compounds in Agaricus blazei murrill


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An efficient microwave-assisted extraction (MAE) technique was employed in the extraction of phenolic compounds from Agaricus blazei murrill, and phenolic compounds were quantified by High-performance liquid chromatography (HPLC). The MAE procedure was optimised, validated and compared with other conventional extraction techniques. MAE gave the best result because of the highest extraction efficiency within the shortest extraction time. The optimal conditions of MAE were 60% ethanol, ratio of solid/liquid 1:30, temperature 110 °C, irradiation power 500 W and three extraction cycles, each 5 min. This is the first report on combining MAE with HPLC for the extraction and quantification of phenolic compounds in A. blazei murrill. The developed MAE method provided a good alternative for the extraction of phenolic compounds in A. blazei murrill as well as other materials.